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Cord Blood CD34+ Stem/Progenitor Cells, Pooled Donors, Frozen
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Description
CD34 is a glycosylated transmembrane protein and represents a well-established marker for human hematopoietic stem and progenitor cells in peripheral blood, bone marrow, and cord blood. CD34+ cells are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system through a process called hematopoiesis. As hematopoietic stem cells progress through hematopoiesis they generate the myeloid (monocytes, macrophages, granulocytes, megakaryocytes, dendritic cells, erythrocytes) and lymphoid (T cells, B cells, NK cells) lineages. The highly specialized cells that arise from hematopoietic stem cells work collaboratively in defending the body against infection and disease.
Human cord blood CD34+ stem and progenitor cells are positively selected using immunomagnetic anti-CD34 (clone QBEND10) microbeads from the mononuclear cell fraction. Pooled CD34+ cells are from two or more donors and are available in larger lots. Isolated cells are characterized by flow cytometry prior to cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Additional information
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Certificate of Analysis
Material Safety Data Sheet
Cord Blood CD34+ Stem Cells, Pooled Donors – Frozen
Protocols
Publications
- Sugimura et al. (2019) Angiocrine Factors from HUVECs Amplify Erythroid Cells. bioRxiv 837823; doi: https://doi.org/10.1101/837823. Article
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Figure 1. Representative flow data of the CD45 frequency, viability, and purity of enriched CD34+ cells from cord blood prior to cryopreservation.
