Bone Marrow Myeloid Cells, Frozen

Description

The antigens CD33 and CD66 are expressed on bone marrow derived myeloid cells such as myeloid progenitor cells, monocytes/macrophages and granulocytes (neutrophils, basophils, eosinophils). These cells play key roles in the adaptive and innate immune responses.

Bone marrow mononuclear cells (MNCs) are separated from whole bone marrow by a density gradient centrifugation protocol. Myeloid cells are obtained by positive selection using anti-CD33 immunomagnetic beads from the MNC population. Isolated cells are characterized by the expression of CD33, CD66b and CD14 from flow cytometry prior to cryopreservation.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.