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Description
Human CD14+ monocytes originate in the bone marrow and move via the bloodstream to infected or damaged peripheral tissues. Once at the tissue, monocytes differentiate into specific tissue macrophage or dendritic cells. These cells are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity.
Human cord blood CD14+ monocytes are positively selected using immunomagnetic anti-CD14 microbeads from the mononuclear cell fraction. Isolated cells are characterized by flow cytometry prior to cryopreservation to ensure a highly pure and viable cell population.
Cord blood is collected from mothers that are negative for HIV, HepB, and HepC during pregnancy. Testing on cord blood can be provided as a custom order.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Additional information
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