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Cord Blood CD34+ Stem/Progenitor Cells, Single Donor, Fresh
CD34 is a glycosylated transmembrane protein and represents a well-established marker for human hematopoietic stem and progenitor cells in peripheral blood, bone marrow, and cord blood. CD34+ cells are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system through a process called hematopoiesis. As hematopoietic stem cells progress through hematopoiesis they generate the myeloid (monocytes, macrophages, granulocytes, megakaryocytes, dendritic cells, erythrocytes) and lymphoid (T cells, B cells, NK cells) lineages. The highly specialized cells that arise from hematopoietic stem cells work collaboratively in defending the body against infection and disease.
Human cord blood CD34+ stem and progenitor cells are positively selected using immunomagnetic anti-CD34 (clone QBEND10) microbeads from the mononuclear cell fraction. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
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- Cleyrat et al. (2017) Gene Editing Rescue of a Novel MPL Mutant Associated with Congenital
Amegakaryocytic Thrombocytopenia. Blood Advances 1: 1815-1826. doi.org/10.1182/bloodadvances.201600291. Abstract
- Takahashi et al. (2018) Enhanced Antibody Responses in a Novel NOG Transgenic Mouse with Restored Lymph Node Organogenesis. Front. Immunol 1(17). doi.org/10.3389/fimmu.2017.02017. Abstract
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Figure 1. Representative flow data of the CD45 frequency, viability, and purity of enriched CD34+ cells from cord blood prior to cryopreservation.