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Cord Blood CD34+ Stem/Progenitor Cells, Single Donor, Fresh

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Description

CD34 is a glycosylated transmembrane protein and represents a well-established marker for human hematopoietic stem and progenitor cells in peripheral blood, bone marrow, and cord blood. CD34+ cells are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system through a process called hematopoiesis. As hematopoietic stem cells progress through hematopoiesis they generate the myeloid (monocytes, macrophages, granulocytes, megakaryocytes, dendritic cells, erythrocytes) and lymphoid (T cells, B cells, NK cells) lineages. The highly specialized cells that arise from hematopoietic stem cells work collaboratively in defending the body against infection and disease.

Human cord blood CD34+ stem and progenitor cells are positively selected using immunomagnetic anti-CD34 (clone QBEND10) microbeads from the mononuclear cell fraction. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Additional information

Anticoagulant

Format

Grade

Species

Cell and Tissue Source

Disease State

Donor Attributes

Product Information Sheet

CB CD34 Single – Fresh

Certificate of Analysis

Sample CoA

Material Safety Data Sheet

Cord Blood – Fresh

Publications

  1. Halkias et al. (2015) Conserved and Divergent Aspects of Human T-cell Development and Migration in Humanized Mice. Immunol Cell Biol 1-11. doi:10.1038/icb.2015.38. Abstract
  2. Cleyrat et al. (2017) Gene Editing Rescue of a Novel MPL Mutant Associated with Congenital
    Amegakaryocytic Thrombocytopenia    . Blood Advances 1: 1815-1826. doi.org/10.1182/bloodadvances.201600291. Abstract
  3. Takahashi et al. (2018) Enhanced Antibody Responses in a Novel NOG Transgenic Mouse with Restored Lymph Node Organogenesis. Front. Immunol 1(17). doi.org/10.3389/fimmu.2017.02017. Abstract

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Figure 1. Representative flow data of the CD45 frequency, viability, and purity of enriched CD34+ cells from cord blood prior to cryopreservation.

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