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Description
Naïve helper T cells are mature T cells that have left the thymus and have yet to be exposed to an antigen presented on the MHC class II molecule of an antigen-presenting cell (APC). In humans, CD4+ naïve helper T cells are constantly recirculating into the secondary lymphoid tissues in order to optimize the opportunity of finding their specific antigen.
Human cord blood CD4+CD45RA+ naïve helper T cells are enriched by depleting cells expressing CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, CD45RO, TCRγ/δ, anti-HLA-DR, and CD235a from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+CD45RA+ naïve helper T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Additional information
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