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Mobilized Leukopaks, G-CSF, Fresh
Human mobilized peripheral blood Leukopaks provide a rich source of hematopoietic stem cells as compared to traditional Leukopaks. This allows researchers greater reproducibility of experiments from a single donor (minimize donor-to-donor variation), increase in scalability of experiments from a single product, and a decrease in same cell variability by obtaining high yields of specific cell types.
Mobilized Leukopaks are collected from healthy IRB consented donors that are injected with a combination of 10µg/kg/day of Granulocyte-Colony Stimulating Factor (G-CSF), which stimulates the bone marrow to produce hematopoietic stem cells and mobilizes them into the bloodstream, prior to the first day of collection. Mobilized mononuclear cells are collected by a trained technician using the Spectra Optia® Apheresis System one and two days post injection. After collection, mobilized Leukopaks are checked by flow analysis to verify the percentage of granulocytes, mononuclear cells, CD34+ cells, and cell viability.
• 2x10e10 TNCs/1 bag
• Enriched PBMCs
• Enriched CD34+ cells
• Low hematocrit
• Low granulocytes
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
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