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Peripheral Blood CD19+CD27+ Memory B Cells, Fresh
Peripheral blood CD19+CD27+ memory B cells become active in the wake of a primary infection from a particular antigen. Memory B cells can persist for years or even a lifetime after an encounter with an antigen. The presence of these cells allows for a faster antibody response if the host comes into contact with the same pathogen.
Human CD19+CD27+ memory B cells are isolated from peripheral blood mononuclear cells. Cells expressing CD2, CD14, CD16, CD36, CD43, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving an enriched CD19+ fraction. CD27+ cells are then enriched from the CD19+ fraction using immunomagnetic anti-CD27 particles leaving purified CD19+CD27+ memory B cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
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Figure 1. Representative flow data of peripheral blood enriched CD19+CD27+ memory B cells prior to cryopreservation.