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Peripheral Blood Eosinophils, Frozen
Eosinophils are proinflammatory granulocytes that arise from the bone marrow and spend only a few days in the peripheral blood and tissues. These cells contain numerous granules in their cytoplasm, which contain proteins that contribute to the role of eosinophils in allergic immune responses as well as parasitic infection. Eosinophils can activate T cells directly by serving as antigen-presenting cells (APCs) and indirectly by secreting a variety of cytokines.
Human peripheral blood eosinophils are isolated from whole peripheral blood in a multi-step process. First, granulocytes are enriched via density gradient centrifugation and processed to remove remaining red blood cells. Finally, non-eosinophils are labeled and removed from the enriched granulocyte population leaving purified, untouched peripheral blood eosinophils. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
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